It takes only 30 seconds hands-on time and conjugates are ready to use in less than 20 minutes without loss of antibodies. Goat Anti Rat (IgG) secondary antibody Alexa Fluor® 488 ... PDF BP Fluor 488 Antibody Labeling Kit - broadpharm.com Product overview Streptavidin Alexa Fluor ® 488 Monovalent Antibody Labeling Kit ab272187 is used to label biotinylated antibodies with Alexa Fluor ® 488. PDF ab236553 - Alexa Fluor® 488 Conjugation Kit - Lightning-Link® improving abcam.com and welcome your feedback. General Protocols are available in the Technical Information section on our website. Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The Antibody - Streptavidin Alexa Fluor ® 488 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.. Several antibodies can be labeled with this kit, and its associated kits, and . Alexa Fluor® 488 goat anti- mouse IgG (H+L . improving abcam.com and welcome your feedback. Catalog number. Removal of non-reacted substrate After the labeling reaction you may wish to separate the non-reacted substrate from the labeled SNAP-Tag fusion protein. (B) Appearance of the resin bed after centrifugation in a fixed-angle rotor or swinging bucket rotor. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. Thaw only when ready to use by placing briefly in a 37°C water bath followed by immediate placement on ice. Alexa Fluor™ 488 染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性（pH 值 4 - pH 值 10）。 除反应性染料制剂外，我们还提供可与多种抗体、肽、蛋白、示踪剂和扩增底物偶联并且针对细胞标记和检测进行优化的 Alexa Fluor™ 488 染料 . Because of its optimal photoswitching properties, Alexa Fluor®647 should be used when staining for only . Immediately transfer Labeled Tubulin-Alexa Fluor® 488 to -80°C upon receipt. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all . The addition of the ALEXA Fluor 488 label to Lightning-Link kits now enables easy, direct conjugation of the label to primary antibodies. Learn about our antibody labeling kits and their advantages. View our protocol for Staining Membrane-associated Proteins. Specificity. BP Fluor 488 Antibody Labeling Kit Components Overview BP Fluor 488 (Alexa Fluor® 488 equivalent) is a green fluorescent dye for the 488 nm argon laser line. For IgGs, we find that labeling with 4-9 moles of Alexa Fluor® 488 dye per Lightning-Link allows for conjugation of antibodies and proteins in less than 20 minutes, using a very simple protocol which is free of separation steps, saving researchers time and precious materials. Category. BP Fluor 488 acid. lysines). This is a cell impermeable substrate based on BG-Alexa Fluor 488. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human MMP-14/MT1-MMP Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB9181G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). A10235 Alexa Fluor™ 488 494/519 Green . Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Normal mouse IgG1 Alexa Fluor® 488: sc-3890 is suitable as an unspecific negative control in any experiment with an Alexa Fluor® 488-conjugated IgG1 primary antibody. Left: HeLa cells transiently expressing Spot-Tag?-Lamin B1 were immunostained with Spot-Label? 488 (ebAF488, 1:800). ALEXA FLUOR. Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG 1 labeling kits. SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit 2-6˚C Components Anti-peroxisomal membrane protein 70 (PMP 70) rabbit IgG fraction* (Component A) 50 µL 250 µg/mL solution in PBS, 2 mM sodium azide • 2-6˚C • Protect from light • DO NOT FREEZE When stored as directed, the components are stable for up to 3 months. Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. Many transformed cell lines also secrete TNF-α. Images were acquired with the . 5.7 Excitation and emission peaks for the four labels that are available in different kits: - (ab272187) Alexa Fluor® 488: Ex 495 nm, Em 520 nm - (ab272188) Atto542 : Ex 542 nm, Em 562 nm It has an excitation maximum at 496 nm . Surface Alexa Fluor 488 was determined to be 93% by HPLC analysis. bs-1123R-A488. The image contains overlay of GFAP (green) and nuclei (blue). With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical connections can be reliably studied and compared in the same animal. CF® Dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes). Alexa 488 absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell and tissue staining of multiple proteins with Alexa Fluor ® 594 and Alexa Fluor ® 647 conjugated secondary antibodies. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Conjugated Primary Antibodies. The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. Here we provide a protocol that describes … Thermo Fisher alexa fluor 488 647 protein labeling kit Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . Please ensure that your instrument's configurations (lasers and optical filters) are appropriate for this dye. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. Take look Maybe later Hello. Label. Conjugated with. Spectrally similar to fluorescein, but the signal is less pH-dependent and more photostable. Anti-OVCA1 PAb ALEXA FLUOR 488. Secondary antibodies conjugated with Alexa Fluor ®647 (Invitrogen), ATTO 488 (Jackson Immuno), and Alexa Fluor 568 (Invitrogen) are recommended. Important Features of Protein Labeling Kits: Labeled proteins typically ready to use in 2 hr (~30 min hands-on time) the Alexa 647 dye is pH insensitive between 4 and 10, and un-like Cy™5 dye, resistant to quenching at high degrees of substi-tution. 3 Alexa Fluor 430 433 541 16,000 4 Alexa Fluor 488 495 519 71,000 5 Alexa Fluor 514 517 542 80,000 6 Alexa Fluor 532 532 553 81,000 7 Alexa Fluor 546 556 573 104,000 8 Alexa Fluor 555 555 565 150,000 9 Alexa Fluor 568 578 603 91,300 10 Alexa Fluor 594 590 617 73,000 11 Alexa Fluor 610 612 628 138,000 12 Alexa Fluor 633 632 647‡ 100,000 The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). Molecular weight [M]- was determined by MS to be 785.1 (785.1 expected). . This antibody is expected to exhibit the same species cross-reactivity as the unconjugated TCF1/TCF7 (C63D9) Rabbit mAb #2203. SNAP-Surface ® Alexa Fluor ® 488 is a photostable green fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells. It is suitable for standard fluorescein filter sets. Alexa Fluor®568 can also be substituted with Cy™ 3B when a yellow-absorbing dye is needed. Alexa Fluor 488 Microscale Protein Labeling Kit, supplied by Thermo Fisher, used in various techniques. The Alexa Fluor 647 protein Labeling Kit contains everything that is required to perform three separate labeling reactions and to purify the resulting conjugates. Alexa Fluor . Once the labeled tubulin is approximately halfway thawed, remove from the water bath and thaw to completion with gentle flicking. Alexa Fluor® 488 FluoroNanogold™ reagents consist of affinity-purified Fab' fragments conjugated to both Alexa Fluor® 488 dye (green) and the 1.4 nm Nanogold® particle.1 An Alexa Fluor® 488 FluoroNanogold™ Fab' conjugate is shown in Figure 1. 电话: +1 877 302 8632 传真: +1 888 205 9894 (Toll-free) 电子邮件: orders@antibodies-online.cn Bioz Stars score: 94/100, based on 1 PubMed citations. Take look. abberior STAR 580. . Supplier. improving abcam.com and welcome your feedback. Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. . ZERO BIAS - scores, article reviews, protocol conditions and more Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6127G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). Once fixed, please follow CST protocol for immunostaining. Store at 2°C to 8°C, protected from light. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. Alexa Fluor® 488 dye and fluorescein conjugates of goat anti-mouse IgG antibody F (ab´)2 fragment were used to detect HEp-2 cells probed with human antinuclear antibodies. improving abcam.com and welcome your feedback. In vitro Protein Labeling: Reaction of SNAP-Sur-face Alexa Fluor 488 (10 µM) with purified SNAP-tag protein (5 µM) in vitro, followed by mass spec analysis, indicated an efficiency of labeling of 95%. Alexa Fluor® 488 Protein Labeling Kit | 4 3.3 Calculate the degree of labeling: moles dye per mole protein = A 494 × dilution factor where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. improving abcam.com and welcome your feedback. Have you used Rabbit IgG Alexa Fluor® 488-conjugated Antibody? ALEXA FLUOR 488. BP Fluor 488 (A max 493 nm, E max Labeling protocol. Streptavidin Alexa Fluor ® 488 Monovalent Antibody Labeling Kit ab272187 is used to label biotinylated antibodies with Alexa Fluor ® 488.. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. modern browser such Google Chrome Hello. Kits are available in 12 Alexa Fluor™ dye colors, biotin, the hapten Oregon Green™ 488, fluorescein EX, and Texas Red™ dye. Human Fibrinogen Conjugate Specifications: 1. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). (A) An empty filter showing the separate parts. Take look. I want to track its localisation in cell by labeling it with a succinimidyl ester of Alexa fluor 488. Lightning-Link® Rapid Alexa Fluor 488 Antibody Labeling Kit Summary Applications/Dilutions Application Notes By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. ALEXA FLUOR 488. Additionally . Please read the entire protocol before starting. Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm) . This protocol only provides a guideline and should be modified according to your experimental needs. Alexa Fluor® 488 Conjugation Kit - Lightning-Link® (ab236553) provides a simple and rapid procedure to covalent labeling of antibodies with Alexa Fluor® 488 by targeting primary amine groups (e.g. The Antibody - Streptavidin Alexa Fluor ® 488 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes. Alexa Fluor ® 488 Conjugation Kit / Alexa Fluor ® 488 Labeling Kit ab236553 uses a simple and quick process for Alexa Fluor 488 labeling / conjugation of antibodies. Bring recombinant α-lactalbumin (Sigma L5385), BSA (Sigma A9647), and protein A (Prospec PRO-774) to a concentration of 1 mg/ml in PBS if needed. Nuclei were stained with Hoechst 33342 ( Product # H3570 ), which can significantly reduce conjugates in. Of ammonium ions or primary amines your instrument & # x27 ; s configurations ( lasers optical. Kits and their advantages incubate for 15 minutes at room temperature, then rinse once PBS. To amplify signal and been implicated as regulators of cell proliferation, differentiation, and images were collected 5! 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